Mechanism-guided Directed Evolution
Department of Biochemistry
Limit of tenure: Up to 3 years
Advert Text:
One predoctoral position (leading to the award of a PhD) is available from 1 September 2008 to work on mechanism-guided directed evolution in Florian Hollfelder's group at the Biochemistry Department of Cambridge University.
Applicants should have a first class degree in chemistry, biochemistry or related areas. Practical laboratory experience in internships, project work etc. is advantageous. Skills in mechanistic enzymology, protein engineering, organic synthesis and molecular biology techniques are specific advantages (but not necessarily required). The project is embedded in a European Research Training Network on Directed Evolution of Functional Proteins (ENEFP) that provides a special training programme and exchange periods in other European laboratories (Strasbourg, Louvain-la-Neuve, Lausanne, Israel amongst others), with whom collaborations are envisaged. Applicants must not have been resident in the UK for more than twelve months in the last three years. There are no citizenship restrictions.
Recent Literature:
- V. Stein, I. Sielaff, K. Johnsson and F. Hollfelder (2007), A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution, ChemBioChem, 8(18):2191-4.
- H. Leemhuis, V. Stein, A. D. Griffiths and F. Hollfelder (2005), New Genotype-phenotype Linkages for Directed Evolution of Functional Proteins, Curr Op. Struct. Biol., 15:472-478.
- Courtois, F., Olguin, L.F., Whyte, G., Bratton, D., Huck, W.T., Abell, C., and Hollfelder, F. (2008). An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem 9, 439-446.
- Huebner, A., Olguin, L.F., Bratton, D., Whyte, G., Huck, W.T., de Mello, A.J., Edel, J.B., Abell, C., and Hollfelder, F. (2008). Development of Quantitative Cell-Based Enzyme Assays in Microdroplets. Anal Chem. available online.
- Baxter, N.J., Blackburn, G.M., Marston, J.P., Hounslow, A.M., Cliff, M.J., Bermel, W., Williams, N.H., Hollfelder, F., Wemmer, D.E., and Waltho, J.P. (2008). Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. J Am Chem Soc 130, 3952-3958.
- Baxter, N.J., Olguin, L.F., Golicnik, M., Feng, G., Hounslow, A.M., Bermel, W., Blackburn, G.M., Hollfelder, F., Waltho, J.P., and Williams, N.H. (2006). A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proc Natl Acad Sci U S A 103, 14732-14737.
Applications should contain a CV, copies of the academic record and the names and addresses of two or more referees, and should be sent by email to jobs@bioc.cam.ac.uk
Quote Reference: PH03463, Closing Date: 15 June 2008
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